Training opportunities in preclinical cell and gene therapy of hemophilia A in the laboratories of Drs. Spencer and Doering are available. Specific aspects of the available training include i) recombinant factor VIII production, ii) recombinant lentiviral vector production and titering, iii) human and murine hematopoietic stem and progenitor cell isolation, ex vivo manipulation, transduction and transplantation into hemophilia A mice and immunocompromised mouse models, respectively, and iv) nonclinical hemostasis assessment vial tail clip or saphenous vein bleeding models.
Dr. Lollar supervises the operation of the Beckman Coulter XL-I analytical ultracentrifuge at Emory University and is available for training in this area of research. Analytical ultracentrifugation is a first-principles physical method that allows real-time observation of the sedimentation of macromolecules in a centrifugal field. Recent computational advances in the method have made it increasingly useful in the characterization of macromolecular interactions, including self-association, hetero-associations and aggregation.
Dr. Li supervises the operation of the mass spectrometry laboratory in the Aflac Cancer and Blood Disorders Center. Hydrogen-deuterium exchange (HDX) mass spectrometry provides information regarding tertiary structure and binding interfaces of proteins based on solvent accessibility. User training is available at Emory University.